Table I.
Comparison of precision for intensity, lifetime, and moment measurements. Dyes were dispensed in uniform volume in microplates to measure signal precision across wells. Lifetime precision (coefficient of variation, CV = SD/mean) is superior to intensity precision in all but the shortest lifetime dye. Further enhancement of precision is seen in the adjusted first moment.
| Intensity | Lifetime | Lifetime | Lifetime | Moment | Moment SD | Moment | |
|---|---|---|---|---|---|---|---|
| CV | (ns) | SD (ns) | CV | (ns) | (ns) | CV | |
| Rose Bengal | 7.17% | 0.040 | 0.011 | 27.50% | 1.187 | 0.004 | 0.34% |
| Rhodamine B | 3.47% | 1.566 | 0.010 | 0.67% | 2.476 | 0.009 | 0.37% |
| Rhodamine 6G | 6.99% | 3.914 | 0.011 | 0.27% | 3.357 | 0.008 | 0.25% |
| EDANS | 2.74% | 12.684 | 0.028 | 0.11% | 10.197 | 0.014 | 0.14% |