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Cks consensus sites in Wee1 are required for Cdk1 inhibitory phosphorylation. Yeast expressing wild-type and mutant (T121S, T196S, T373S) Wee1 (Swe1) were released from a G1 arrest. Cells at the indicated time points were lysed and Swe1 (a) and phosphorylation on Cdk1 Y19 (b) were probed. Exposures of the full gels for this experiment can be seen in Supplemental Figure 7c.
