Fig. 6.
Cryo-preparations. (A) A large vascular bundle in a frost-frozen petiole of a poppy preparation as the one in Fig. 3A. The bundle is surrounded by collapsed parenchyma cells and all the xylem vessels are ice-filled. Bar = 50 μm. (B) A similar vascular bundle in a thawed petiole as seen in Fig. 3B. The surrounding parenchyma cells are rehydrated and many of the xylem vessels are embolized (they appear white because of the ice chips that lodge in the spaces during planing). Bar = 50 μm.