Table 1.
Description of microsatellite markers, motifs, chromosome map location and fragment size reported by the authors (GWM, Röder et al., 1998; GDM, Pestsova et al., 2000; PSP, Bryan et al., 1997)
| Micro-satellite | Published information |
‘Barbela’ lines from this studies |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Motifs |
Chromosome location |
Fragment size (bp) (cultivar)* |
Fragment size (bp) |
No. of alleles |
PIC |
|||||
| GWM3 | (CA)18 | 3DL | − (S); 84 (O) | 74–110 | 6 | 0·26 | ||||
| GWM30 | (AT)19 (GT)15 | 3AS | 205 (S); 196 (O) | 209–281 | 5 | 0·68 | ||||
| GWM46 | (GA)2 GC (GA)33 | 7BL | 179 (S); 186 (O) | 154–160 | 2 | 0·14 | ||||
| GDM93† | (GA)16 | 2A | 134 (S); – (O) | 100–128 | 7 | 0·56 | ||||
| 4B | 215 (S); 217 (O) | 217–225 | 3 | 0·17 | ||||||
| GWM106 | (GA)24 | 1DS | 81 (S); – (O) | 121–139 | 4 | 0·59 | ||||
| GWM111 | (CT)32 (GT)17 | 7DL | 184 (S); 206 (O) | 206–216 | 5 | 0·52 | ||||
| GWM154 | (GA)37imp | 5AS | 120 (S); 102 (O) | 102–128 | 4 | 0·68 | ||||
| GWM161 | (CT)15 | 3DS | 145 (S); 154 (O) | 148–154 | 3 | 0·49 | ||||
| GWM192 | (CT)46 | 5DS | 232 (S); 191 (O) | 190–210 | 4 | 0·55 | ||||
| GWM272 | (CA)17 | 5DL | 140 (S); 138 (O) | 140–148 | 2 | 0·50 | ||||
| GWM276 | (CT)24 | 7AL | 101 (S); 109 (O) | 118–139 | 9 | 0·81 | ||||
| GWM301 | (GA)31 (G)12 | 2DL | 210 (S); – O | 196–226 | 6 | 0·76 | ||||
| GWM304 | (CT)22 | 5AS | 208 (S); 202 (O) | 200–206 | 5 | 0·71 | ||||
| GWM311 | (GA)29 | 2AL | 120 (S); – (O) | 120–136 | 7 | 0·61 | ||||
| 2DL | 143 (S); 157 (O) | 143–171 | 4 | 0·51 | ||||||
| GWM320 | (GT)9 (GA)15 | 2DL | 226 (S) | 224–232 | 5 | 0·65 | ||||
| GWM349 | (GA)34 | 2DL | 243 (O) | 243–275 | 6 | 0·72 | ||||
| GWM361 | (GA)20imp | 6BS | 123 (S); 125 (O) | 131–139 | 3 | 0·63 | ||||
| GWM382 | (GA)26 | 2AL | 86 (S) | 86–96 | 3 | 0·45 | ||||
| 2BL | 184 (S) | 184–190 | 3 | 0·61 | ||||||
| 2DL | 108 (S) | 108–134 | 3 | 0·46 | ||||||
| GWM539 | (GA)27 | 2DL | 157 (S); 143 (O) | 109–159 | 9 | 0·86 | ||||
| GWM570 | (CT)14 (GT)18 | 6AL | 143 (S); 149 (O) | 136–158 | 7 | 0·76 | ||||
| GWM610 | (GA)17imp | 4AL | 162 (S); 172 (O) | 164–200 | 9 | 0·82 | ||||
| GWM645 | (CT)23imp | 3DL | 145 (S); 161 (O) | 135–161 | 6 | 0·70 | ||||
| PSP2999 | (CAG)5 (CAA)8 | 1AS | 150 (CS) | 138–173 | 11 | 0·74 | ||||
| PSP3000 | (CAA)15 | 1BS | 286 (CS) | 282–290 | 3 | 0·14 | ||||
| PSP3029 | (AG)17 | 2AL | 150 (CS) | 144–150 | 2 | 0·10 | ||||
| 6AL | 166 (CS) | 166–170 | 2 | 0·06 | ||||||
| 6AS | 245 (CS) | 241–253 | 6 | 0·60 | ||||||
| PSP3030 | (AG)15 | 4BL | 208 (CS) | 212–220 | 3 | 0·28 | ||||
| 3BS | 172 (CS) | 174–178 | 2 | 0·16 | ||||||
| PSP3050 | (CT)12 (AT)13 | 7AS | 160 (CS) | 160–170 | 3 | 0·54 | ||||
For the 59 ‘Barbela’ lines, fragment size range, number of alleles detected and polymorphism information content (PIC, see text) are given.
*
S, Synthetic hexaploid wheat; O, ‘Opata’; CS, ‘Chinese Spring’; –, null allele.
†
Two loci were not mapped to a specific arm.