Table 5.
Fluorescence signal intensity*on meiotic chromosome of the triploid hybrid (clone 12-28) from the H. monacanthus and H. megalanthus cross, with different ratios of tester DNA (labelled DNA probe) to block DNA (unlabelled DNA) in GISH analysis
| Tester : block ratio |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source of tester DNA |
Source of block DNA |
1 : 2 |
1 : 10 |
1 : 16 |
1 : 50 |
1 : 100 |
1 : 200 |
|||||
| H. monacanthus | H. megalanthus | +++ | +++ | ND | + | ND | ND | |||||
| H. megalanthus | H. monacanthus | ND | ND | + | ND | ND | ND | |||||
| H. monacanthus | Salmon sperm DNA | ND | +++ | ND | ++ | + | 0 | |||||
| H. megalanthus | Salmon sperm DNA | ND | +++ | ND | ND | + | ND | |||||
ND, Not determined.
*
+, ++, +++, Arbitrary grades; 0, no signal detected.