Table 1.
Cell numbers of various subsets in lymphoid tissues from WT, FcμR(+) and FcμR(−) B6/lpr mice
| WT | FcμR(+) B6/lpr | FcμR(−) B6/lpr | Mann–Whitney U-test (P) | Fold increase | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| n = 5 | n = 5 | n = 5 | WT versus FcμR(+)lB6/lpr | WT versus FcμR(−) B6/lpr | FcμR(+) versus FcμR(−) B6/lpr | FcμR(+) B6/lpr versus WT | FcμR(−) B6/lpr versus WT | ||||
| Mean | 1 SD | Mean | 1 SD | Mean | 1 SD | ||||||
| Spleen | |||||||||||
| Total cell number (×106) | 108.6 | 23.4 | 308.4 | 186.7 | 194.5 | 50.4 | <0.01 | <0.05 | NS | 2.8 | 1.8 |
| B cells (CD19+) | 58.8 | 15.3 | 135.9 | 69.0 | 68.1 | 15.6 | <0.01 | NS | <0.05 | 2.3 | 1.2 |
| B2 (CD19+/CD5−/CD11b−) | 56.1 | 14.4 | 124.3 | 61.8 | 56.2 | 11.1 | <0.05 | NS | <0.01 | 2.2 | 1.0 |
| B1a (CD19+/CD5+/CD11b+) | 1.4 | 0.5 | 3.1 | 1.5 | 2.3 | 0.7 | <0.05 | NS | NS | 2.3 | 1.7 |
| B1b (CD19+/CD5−/CD11b+) | 0.9 | 0.4 | 7.2 | 5.3 | 8.4 | 6.6 | <0.01 | <0.01 | NS | 8.0 | 9.5 |
| FO (CD21+/CD23+) | 44.6 | 11.3 | 94.4 | 54.6 | 44.6 | 8.8 | <0.05 | NS | <0.05 | 2.1 | 1.0 |
| MZ (CD21hi/CD23−) | 8.6 | 1.0 | 15.8 | 2.2 | 2.0 | 0.5 | <0.01 | <0.01 | <0.01 | 1.8 | 0.2 |
| NF (CD21−/CD23−) | 4.6 | 1.0 | 25.0 | 19.6 | 17.9 | 13.0 | <0.01 | <0.05 | NS | 5.4 | 3.9 |
| Plasma cells (CD138+/CD19−) | 0.25 | 0.1 | 25.6 | 23.3 | 10.1 | 9.4 | <0.01 | <0.01 | NS | 102.3 | 40.4 |
| T cells (CD3+) | 27.6 | 7.5 | 97.3 | 73.8 | 71.3 | 24.0 | <0.01 | <0.01 | NS | 3.5 | 2.6 |
| Normal T (CD3+/B220−) | 26.3 | 7.3 | 46.7 | 34.1 | 45.3 | 9.8 | NS | <0.05 | NS | 1.8 | 1.7 |
| CD4+ T | 13.2 | 4.8 | 28.8 | 28.5 | 24.7 | 14.3 | NS | NS | NS | 2.2 | 1.9 |
| CD8+ T | 11.2 | 2.7 | 11.6 | 5.6 | 16.2 | 9.6 | NS | NS | NS | 1.0 | 1.4 |
| Atypical T (CD3+/B220+) | 1.0 | 0.4 | 47.4 | 38.3 | 24.0 | 15.5 | <0.05 | <0.05 | NS | 45.2 | 22.9 |
| DN (CD3+/CD4−/CD8−) | 0.3 | 0.1 | 38.8 | 29.6 | 20.2 | 13.1 | <0.01 | <0.01 | NS | 117.1 | 60.9 |
| Lymph nodes | |||||||||||
| Total cell number (×106) | 10.3 | 5.5 | 786.4 | 937.3 | 295.3 | 233.8 | <0.01 | <0.01 | NS | 76.6 | 28.8 |
| B cells (CD19+) | 3.8 | 2.4 | 96.2 | 58.1 | 76.2 | 61.1 | <0.01 | <0.01 | NS | 25.4 | 20.1 |
| B2 (CD19+/CD5−/CD11b−) | 3.6 | 2.2 | 87.5 | 51.8 | 67.9 | 54.3 | <0.01 | <0.01 | NS | 24.5 | 19.0 |
| B1a (CD19+/CD5+/CD11b+) | 0.1 | 0.1 | 3.3 | 1.5 | 3.3 | 2.4 | <0.01 | <0.01 | NS | 23.8 | 23.7 |
| B1b (CD19+/CD5−/CD11b+) | 0.04 | 0.03 | 4.4 | 4.6 | 3.9 | 4.0 | <0.01 | <0.01 | NS | 101.1 | 89.5 |
| Plasma cells (CD138+/CD19−) | 0.03 | 0.01 | 108.4 | 147.9 | 39.2 | 54.2 | <0.01 | <0.01 | NS | 3838.9 | 1388.6 |
| T cells (CD3+) | 5.4 | 3.0 | 585.3 | 785.6 | 161.0 | 130.8 | <0.01 | <0.01 | NS | 107.8 | 29.7 |
| Normal T (CD3+/B220−) | 5.3 | 3.0 | 109.0 | 45.1 | 68.1 | 36.5 | <0.01 | <0.01 | NS | 20.6 | 12.9 |
| CD4+ T | 2.5 | 1.5 | 61.0 | 40.9 | 30.9 | 17.8 | <0.01 | <0.01 | NS | 23.9 | 12.1 |
| CD8+ T | 2.6 | 1.4 | 33.8 | 9.1 | 27.6 | 13.7 | <0.01 | <0.01 | NS | 13.1 | 10.7 |
| Atypical T (CD3+/B220+) | 0.09 | 0.07 | 413.0 | 634.2 | 89.5 | 95.8 | <0.01 | <0.01 | NS | 4437.3 | 961.3 |
| DN (CD3+/CD4−/CD8−) | 0.03 | 0.01 | 375.6 | 587.6 | 79.0 | 84.1 | <0.01 | <0.01 | NS | 14472.3 | 3044.7 |
| Bone marrow | |||||||||||
| Total cell number (×106) | 15.3 | 2.4 | 17.1 | 3.3 | 14.3 | 1.7 | NS | NS | NS | 1.1 | 0.9 |
| B-lineage cells (CD19+) | 2.1 | 0.2 | 2.7 | 0.8 | 1.5 | 0.2 | NS | <0.01 | <0.01 | 1.3 | 0.7 |
| Plasma cells (CD138+/CD19−) | 0.03 | 0.01 | 0.05 | 0.02 | 0.03 | 0.01 | <0.05 | NS | NS | 1.6 | 1.0 |
NS, not significant; SD, standard deviation.