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. 2004 May;93(5):581–590. doi: 10.1093/aob/mch082

Table 1.

cDNA clones used as probes in northern blot hybridizations

cDNA Clone/Genbank accession no. Putative function or closest homologue in databases; previous reports of expression pattern in grapevine roots (see references) Source/reference
Stilbene synthase VvStSy pSV696/X76892 Catalyses synthesis of resveratrol and derivatives; induced in cell cultures by fungal elicitation; expression in grapevine roots unknown, but constitutive in grapevine seedlings H. Kindl, University of Marburg, Germany. (Melchior and Kindl, 1991; Sparvoli et al., 1994)
Chalcone synthase VvCHS pBS305/X75969 First dedicated enzyme in flavonoid synthesis pathway; expression in grapevine roots unknown F. Sparvoli, Universita degli Studi di Milano, Milan, Italy. (Sparvoli et al., 1994; Boss et al., 1996)
Phenylalanine ammonia‐lyase VvPAL pBS204/X75967 Key regulatory enzyme in phenylpropanoid pathway; upregulated in V. vinifera on fungal elicitation; expression in grapevine roots unknown, but constitutive in grapevine seedlings F. Sparvoli, Universita degli Studi di Milano, Milan, Italy. (Sparvoli et al., 1994; Boss et al., 1996)
Polyphenol oxidase GPO1 pID96/A27657 Polyphenol oxidase from grape berries; expressed at high levels in young grapevine roots, but not mature tissues I. Dry, CSIRO, Adelaide, Australia. (Dry and Robinson, 1994)
Thaumatin‐like protein VvTL1 pTL3/AF003007 Thaumatin‐like protein; not expressed in grapevine roots D. Tattersall, University of Adelaide, Adelaide, Australia. (Tattersall et al., 1997)
Thaumatin‐like protein VvTL2 Y10992 Thaumatin‐like protein. Partial homology to osmotin‐like protein from V. vinifera; expression in roots unknown I. Dry, CSIRO, Adelaide, Australia. (Jacobs et al., 1999)
PR‐4 protein VvPR4a pWIN52/AF061329 PR4‐like protein; not expressed in grapevine roots D. Tattersall, University of Adelaide, Adelaide, Australia. (D. Tattersall, pers. comm.)
Grip 15 AJ237984 Partial homology to nodule cell wall protein from Maakia amurensis; not expressed in grapevine roots C. Davies, CSIRO, Adelaide, Australia. (Davies and Robinson, 2000)
Grip 28 AJ237985 Similarity to pectin methylesterases; partial homology to protein from alfalfa nodules; not expressed in grapevine roots C. Davies, CSIRO, Adelaide, Australia. (Davies and Robinson, 2000)
Grip 31 AJ237986 Partial homology to protein in young nodule pericycle and in older senescent nodule cells in Alnus glutinosa; expressed in grapevine roots at a low level C. Davies, CSIRO, Adelaide, Australia. (Davies and Robinson, 2000)
Grip 68 AJ237987 Partial homology to Grip 31, homology to protein from potato stolon tip; expressed in grapevine root at a low level C. Davies, CSIRO, Adelaide, Australia. (Davies and Robinson, 2000)
Grip 21 AJ237988 Related to proteins produced in glucose starvation response in maize roots; expressed in grapevine root at a low level C. Davies, CSIRO, Adelaide, Australia. (Davies and Robinson, 2000)
Sucrose transporter 1/2 VvSuc12 AF021809 Sucrose transporter from grape berries; homology to maize sucrose transporter; expression in grapevine roots unknown C. Davies, CSIRO, Adelaide, Australia. (Davies et al., 1999)
Sucrose transporter 2/7 VvSuc27 AF021810 Sucrose transporter from grape berries; homology to maize sucrose transporter; expression in grapevine roots unknown C. Davies, CSIRO, Adelaide, Australia. (Davies et al., 1999)

All clones are from Vitis spp.