Table 1.

Primers used for real-time quantitative polymerase chain reaction

Gene	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)
Ckip-1	GAGCTTTCGGGTCGATCTGG	GGCTCCCTTGTCTGGTCTTT
sFlt-1	TGACATACCCAAACTCGTG	AAAGCCTCTCCTACTGTCC
Alp	GGCTCCCTTGTCTGGTCTTT	GGACGCCGTGAAGCAGGTGA
VEGF	CAGCTATTGCCGTCCAATTGA	CCAGGGCTTCATCATTGCA
OCN	GGTGCAGACCTAGCAGACACCA	AGGTAGCGCCGGAGTCTATTCA
GAPDH	CAAGTTCAACGGCACAGTCA	CCATTTGATGTTAGCGGGAT

Abbreviations: Ckip-1, casein kinase 2 interaction protein 1; sFlt-1, soluble VEGF receptor 1; Alp, alkaline phosphatase; VEGF, vascular endothelial growth factor; OCN, osteocalcin; GADPH, glyceraldehyde-3-phosphate dehydrogenase.