Figure 1.
(A) A schematic that depicts the gross structural organization of the Gram negative outer membrane. (B) Silver stained SDS PAGE analysis of LPS from the K. pneumoniae ATCC 13883 strains examined in this study. Increasing sample amounts (0.5, 1.0, 1.5 μg) were loaded per lane to allow for visualization on minor components and owing to the differential staining of each sub structure. The position of each LPS sub structural component is indicated on the right ordinate. Molecular mass standards are shown on the left ordinate, in lane 1. The LPS of E. coli serotype 0111:B4 (Sigma Aldrich) is shown as a reference in lane 2. The dashed line indicates the mass shift of the lipid A inner core between the paired strains. (Left panel) A schematic that depicts the reported sub structures for K. pneumoniae serotype O2 LPS and K. pneumoniae lipid A (variable fatty acyl positions are indicated in bold and dashed line).