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(A) NPN uptake kinetics of a suspension of mid logarithmic phase K. pneumoniae cells. (B) Effects of increasing concen trations polymyxin B and colistin on the uptake of NPN into the outer membrane of K. pneumoniae ATCC 13883 and (C) polymyxin resistant K. pneumoniae ATCCR. (D) The normalized peak NPN fluorescence is plotted as a function of the polymyxin B and colistin concentration. K. pneumoniae ATCC 13883, polymyxin B (blue, ▲); K. pneumoniae ATCC 13883, colistin (red, ◇).
