Fig. 5.

Acot2 facilitates FAO and increases proton leak in liver mitochondria. A: State 3 oxygen consumption rate (JO₂) which is the maximal ADP-stimulated JO₂, RCR (state 3/state 4 which is the maximal JO₂ in the presence of oligomycin), and proton-motive force (delta psi) in liver mitochondria from fed mice supplied with saturating pyruvate/malate (10 mM/5 mM) and ADP. Oligomycin was used to obtain the state 4 rate (not shown). A.U., arbitrary units. n = 5/group. B: Citrate synthase in liver mitochondria from fed mice. C–E: JO₂ measured following addition of saturating ADP, then FA substrate, then oligomycin (in that order). Malate was already present when mitochondrial suspensions were added to the Seahorse plate. RCR: ratio of JO₂ in the presence of malate + ADP + FA to JO₂ in the presence of malate + ADP + FA + oligomycin. *P = 0.05, **P = 0.01, ***P < 0.002; unpaired t-test; n = 6–8/group. F: Proton-motive force. **P < 0.02, unpaired t-test, n = 5/group. A–F: Black symbols/bars, Ad-Ctrl; open bars/symbols, Ad-Acot2.