Fig. 2.

Regulation of ATGL, COX-1, COX-2, and HPGDS protein levels in immunologically activated human MCs. IgE-dependent MC activation was performed as described in Fig. 1. MCs were harvested after the indicated periods of activation, and protein levels were analyzed by Western blotting. Fifty micrograms of total protein lysate per lane was separated by 10% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and probed with specific antibodies against ATGL (A) and COX-1, COX-2, or HPGDS (B). GAPDH was used as a loading control.