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SP-HPLC analyses for products (analyzed as methyl esters) from incubation of platelet 12-LOX with 14S,15R-EETs (A) and 14R,15S-EETs (B) after 10 and 30 min, and the products from incubation of human 15-LOX-1 with 14S,15R-EET (C). (Incubation of 15-LOX-1 with 14R,15S-EET is given in the supplementary Fig. V.) Chromatographic conditions were as in Fig. 2. The structures of the products are shown in Scheme 2.
