Figure 3.

The effect of fluoxetine on proliferation and migration of 231BR cells. A) Fluoxetine treatment did not affect 231BR proliferation in vitro at 24, 48, and 72 h of incubation, except at 5000 ng/ml, when the drug caused cell growth arrest, p < 0.001, 2-way ANOVA with Bonferroni post-hoc analysis. n = 6-8. B) Migratory ability of 231BR cells in vitro was not affected by fluoxetine treatment, p = 0.98, one-way ANOVA, with Dunnett’s multiple comparison test. n = 8. C) At high doses, fluoxetine produces cytotoxic effect on 231BR breast cancer cells. Cell numbers were obtained after 24, 48, and 72 h of incubation with fluoxetine, as described. Whereas incubation with 5000 ng/ml (5 μg/ml) of fluoxetine caused an arrest in cellular proliferation starting at 48 hours, higher fluoxetine doses - 100 μg/ml, 500 μg/ml, and 1000 μg/ml - exhibited a clear toxic effect on 231BR cells. n = 4-7. Two independent experiments were conducted for each assay.