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. Author manuscript; available in PMC: 2014 Nov 24.
Published in final edited form as: Curr Protoc Neurosci. 2013 Oct 23;4(435):4.35.1–4.35.20. doi: 10.1002/0471142301.ns0435s65

Figure 1.

Figure 1

Combinatorial viral delivery to study pathway specific gene function. (A) Illustration of combined viral injections of a conditional AAV-FLEX construct (red) into a region containing a neuronal population of interest and a CAV2-Cre viral vector (blue) into a projection target of the neurons of interest. (B) Illustration of local (red) AAV-FLEX viral vector transduction and retrograde (blue) CAV2-Cre transduction. (C) Demonstration of combinatorial viral-mediated gene expression. Injection of CAV2-Cre into a target region of interest (blue) of a Cre-dependent reporter line (Ai14) illustrates coverage area of viral vector injection and the number of neurons retrogradely transduced. Injection of AAV-FLEX containing conditional expression cassette of interest locally transduces neurons at the sight of injection; however, only neurons with combined CAV2-Cre and AAV-FLEX will express the transgene (red).