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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Curr Protoc Nucleic Acid Chem. 2014 Mar 26;5(514):5.14.1–5.14.15. doi: 10.1002/0471142700.nc0514s56

Figure 2.

Figure 2

Synthesis of N2-[3-[(trifluoroacetyl)amino]propyl]-5′-O-DMT-O6-(trimethylsilylethyl)-3′-O-[(2-cyanoethyl-N,N′-diisopropyl)phosphoramidite]-2′-deoxyguanosine. a) Ethyl trifluoroacetate and methanol, room temperature, 4 hours; b) 4,4′-dimethoxytrityl chloride, pyridine, room temperature, 8 hours; c) 2-cyanoethyl N,N,N,N-tetraisopropylphosphane, tetrazole, dichloromethane, room temperature, 3 hours.