Figure 4. Configuration of templates used for evaluating the sensitivity of SIBA for point mutation(s).

Eight additional templates harboring different numbers of point mutation(s) 1–4 were synthesized. The point mutation(s) were either located in regions homologous to the IO DNA or the IO 2′-O-methyl RNA region (SB-IO). The black box on the templates indicates the location of the point mutation (s).