Table 1. Sensitivity of SIBA extension to point mutations.

	No. of point mutations
	1	2	3	4
Location of point mutation(s) within the amplification template	Δdt (Minutes)
Homologous to the IO DNA region	SB-DNA1	SB-DNA2	SB-DNA3	SB-DNA4
	7.4	24.0	ND	ND
Homologous to the IO 2′-O-methyl RNA region	SB-MET1	SB-MET2	SB-MET3	SB-MET4
	11.7	ND	ND	ND

The SIBA reaction was performed with either a fully homologous target template (SB-template) or with templates containing 1–4 base point mutation(s). The results are expressed as the delay in the threshold detection time (dt), i.e., the average Δdt =  average dt of a template containing point mutation(s) minus the average dt of the fully homologous target template (SB-template). ND denotes no detectable amplification of a template. SB-F21 and SB-R21 were the upstream and downstream primers, respectively. The invasion oligonucleotide (IO) used was SB-IO.