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. 2014 Nov 24;9(11):e113221. doi: 10.1371/journal.pone.0113221

Figure 1. Vectors used for 293T cell transfection.

Figure 1

A: HIV-2CGP, a CMV driven gag-pol expression construct. B: CRU5SINCGW, HIV-2 based minimal vector with a GFP expression cassette. C: pMD.G a plasmid coding for VSV envelope protein. CMV: human cytomegalovirus immediate early promoter; SD: splice donor site; rre: rev response element. BGHpA: bovine growth hormone polyadenylation signal. U3-R-U5; retroviral long terminal repeats. cppt; central polypurine tract. GFP; green fluorescence protein. WPRE; woodchuck hepatitis virus post-transcriptional regulatory element. VSV-G; vesicular stomatitis virus G protein. PolyA; polyadenylation site.