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A: supernatants 1, 2, 3, 4 obtained from steps 1-4 following expression in BL21(DE3) cells. 15 µl of supernatant was run on 16% SDS-polyacrylamide gel, the asterisk indicates the solubilized pellet after 15x dilution with water to assist visualization. B: protease fractions after reversed-phase high performance liquid chromatography, arrows indicate the pooled fractions.
