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. 2014 Nov 24;9(11):e113603. doi: 10.1371/journal.pone.0113603

Figure 4. Abundance of amoA, nirK, nirS and nosZ genes in the four sludge samples revealed by qPCR (A and B) and metagenomic (alignment) approaches (C and D).

Figure 4

A: relative abundance of the genes normalized to the mass of the extracted DNA; B: relative abundance of the genes normalized to the abundance of 16S rRNA gene; C: Number of the Illumina sequencing reads aligned to the genes sequences in the local database at identity cutoff>90% and alignment length>50 bp; D: Relative abundance of the genes in the Illumina dataset of each sludge sample.