Table 1. List of the differentially expressed proteins identified by 2D-DIGE and LC-MS/MS analysis.
Spot numbera | Ratiosb | Protein accession | Protein name | Symbol | Molecular weight (Da) | Number of unique peptides | Number of unique spectra | Number of total spectra | Sequence coverage | ||
LPS/untreated | LPS+AGE/LPS | LPS+FruArg/LPS | |||||||||
607 | −1.69 | 2.04 | 1.68 | IPI00330862 | Ezrin | EZR | 69,408.80 | 31 | 41 | 44 | 47.40% |
654 | −1.38 | −1.22 | 1.18 | IPI00230035 | ATP-dependent RNA helicase DDX3X | ERH | 73,103.70 | 56 | 90 | 156 | 65.10% |
656 | −1.25 | 1.55 | 1.77 | IPI00110588 | Moesin | MSN | 67,768.80 | 56 | 99 | 163 | 73.10% |
772 | −1.84 | 1.82 | 2.35 | IPI00403810 | Tubulin alpha-1C chain | TUBA1C | 49,909.60 | 23 | 43 | 140 | 58.10% |
780 | 1.27 | 1.2 | 1.37 | IPI00310091 | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform | PPP2R1A | 65,323.70 | 37 | 67 | 142 | 62.80% |
782 | −1.47 | −1.03 | 1.1 | IPI00223253, IPI00224575, IPI00890005 | Isoform 1 of Heterogeneous nuclear ribonucleoprotein K | HNRNPK | 51,030.40 | 22 | 41 | 58 | 56.40% |
792 | 1.41 | 3.03 | 1.95 | IPI00126083 | EH domain-containing protein 1 | EHD1 | 60,605.20 | 41 | 70 | 99 | 79.80% |
795 | −1.39 | 1.58 | 1.41 | IPI00228385 | Glucose-6-phosphate 1-dehydrogenase X | G6PD | 59,263.60 | 39 | 59 | 89 | 64.50% |
IPI00323600 | Coronin-1A | CORO1A | 50,988.90 | 29 | 47 | 88 | 53.40% | ||||
810 | 1.69 | −1.96 | −1.38 | IPI00230108 | Protein disulfide-isomerase A3 | PDIA3 | 56,680.40 | 50 | 70 | 107 | 74.90% |
825 | −1.47 | 1.55 | 1.58 | IPI00228385 | Glucose-6-phosphate 1-dehydrogenase X | G6PD | 59,263.60 | 44 | 81 | 271 | 77.90% |
842 | −1.19 | 1.45 | 1.5 | IPI00225961 | D-3-phosphoglycerate dehydrogenase | PHGDH | 56,585.20 | 33 | 50 | 114 | 54.60% |
844 | −1.48 | 1.41 | 1.52 | IPI00230139 | Peptidyl-prolyl cis-trans isomerase FKBP4 | FKBP4 | 51,573.10 | 46 | 84 | 119 | 76.00% |
901 | 2.69 | 1.14 | 1.06 | IPI00111285 | Immune-responsive gene 1 protein | IRG1 | 53,759.60 | 30 | 55 | 104 | 67.60% |
909 | 2.2 | 1.39 | 1.21 | IPI00111285 | Immune-responsive gene 1 protein | IRG1 | 53,759.60 | 32 | 62 | 159 | 68.90% |
913 | 2.64 | 1.41 | 1.27 | IPI00111285 | Immune-responsive gene 1 protein | IRG1 | 53,759.60 | 30 | 57 | 151 | 68.90% |
940 | 1.08 | 1.23 | 1.49 | IPI00125676 | Caspase-1 | CASP1 | 45,642.00 | 27 | 46 | 62 | 53.00% |
IPI00462072 | Alpha-enolase | ENO1 | 47,141.70 | 24 | 40 | 61 | 44.50% | ||||
963 | 2.22 | −1.83 | −1.09 | IPI00133417 | Interferon-induced protein with tetratricopeptide repeats 3 | IFIT3 | 47,223.80 | 44 | 79 | 153 | 83.60% |
965 | −2.17 | 1.32 | 1.43 | IPI00111013 | Cathepsin D | CTSD | 44,955.00 | 22 | 38 | 48 | 52.40% |
997 | 3.82 | −1.87 | −1.54 | IPI00120113 | UMP-CMP kinase 2, mitochondrial | CMPK2 | 50,036.40 | 37 | 59 | 188 | 73.20% |
1064 | 1.29 | 1.42 | 1.33 | IPI00315550 | Glutaredoxin-3 | GLRX3 | 37,778.70 | 24 | 44 | 80 | 53.40% |
IPI00126048 | 26S proteasome non-ATPase regulatory subunit 13 | PSMD13 | 42,810.00 | 23 | 31 | 36 | 54.50% | ||||
1069 | −2.38 | 1.58 | 1.34 | IPI00315550 | Glutaredoxin-3 | GLRX3 | 37,778.70 | 23 | 39 | 86 | 52.20% |
IPI00126048 | 26S proteasome non-ATPase regulatory subunit 13 | PSMD13 | 42,810.00 | 25 | 37 | 45 | 61.70% | ||||
1170 | 1.13 | 1.48 | 1.35 | IPI00314950 | 60S acidic ribosomal protein P0 | RPLP0 | 34,217.50 | 24 | 42 | 136 | 69.70% |
1241 | −2.06 | −1.08 | −1.04 | IPI00122547 | Voltage-dependent anion-selective channel protein 2 | VDAC2 | 31,733.60 | 16 | 22 | 78 | 55.90% |
1274 | −1.42 | 1.02 | −1.33 | IPI00230044 | Isoform 2 of Tropomyosin alpha-3 chain | TPM3 | 29,021.20 | 35 | 67 | 165 | 77.40% |
1315 | 1.38 | −1.03 | 1.42 | IPI00114329 | Glutamate–cysteine ligase regulatory subunit | GCLM | 30,535.20 | 15 | 23 | 46 | 50.00% |
1357 | 1.38 | 1.13 | 1.06 | IPI00457898 | Phosphoglycerate mutase 1 | PGAM1 | 28,832.80 | 22 | 53 | 112 | 78.70% |
1446 | 1.48 | −1.03 | 1.13 | IPI00126042 | Ras-related protein Rab-14 | RAB14 | 23,897.60 | 18 | 23 | 29 | 64.70% |
IPI00132397 | GTP-binding protein SAR1b | SAR1B | 22,382.90 | 12 | 22 | 32 | 60.60% | ||||
IPI00117264, IPI00895414 | Protein DJ-1 | PARK7 | 19,908.10 | 12 | 16 | 24 | 60.80% | ||||
1464 | 1.43 | 1.28 | 1.21 | IPI00555023 | Glutathione S-transferase P 1 | GSTP1 | 23,610.10 | 10 | 17 | 52 | 48.60% |
1474 | 2.78 | −1.76 | −1.35 | IPI00121788 | Peroxiredoxin-1 | PRDX1 | 22,177.50 | 18 | 24 | 39 | 64.30% |
IPI00109109 | Superoxide dismutase [Mn], mitochondrial | SOD2 | 24,603.20 | 12 | 17 | 29 | 44.60% | ||||
1477 | 2.35 | −1.2 | 1.04 | IPI00121788 | Peroxiredoxin-1 | PRDX1 | 22,177.50 | 24 | 43 | 131 | 76.40% |
IPI00109109 | Superoxide dismutase [Mn], mitochondrial | SOD2 | 24,603.20 | 15 | 28 | 113 | 44.60% | ||||
1501 | 1.01 | 1.34 | 1.62 | IPI00121788 | Peroxiredoxin-1 | PRDX1 | 22,177.50 | 26 | 52 | 310 | 80.40% |
1737 | 4.57 | −1.89 | −1.52 | IPI00555085 | Ubiquitin-like protein ISG15 | ISG15 | 17,897.50 | 9 | 21 | 60 | 55.30% |
Protein spot number according to Figure 4.
Average volume ratio based on the spots normalized volume across the four groups was calculated by the SameSpots software. A p value for the one-way analysis of variance <0.05 was accepted.