Figure 5. Inhibitors of STAT3 and RIP1K, but not caspase-1, suppress inflammatory responses to vaccinia virus infection in keratinocytes.

HEK001 and 308 keratinocytes and Vero cells were transfected with reporter plasmids encoding luciferase under the control of promoters for NFκB (A), IFNβ (B), or ISRE (C). Cells were then challenged with UV inactivated ACAM-2000, or with infectious ACAM-2000 at 20 MOI combined with DMSO, Stattic, caspase-1 inhibitor, or Nec-1. Luciferase activity was detected at 24 hours post-infection (n = 4 wells per condition). Representative of triplicate experiments is shown. Asterisk, p≤0.05.