Figure 7. Treatment with Stattic reduces inflammatory responses in keratinocytes exposed to TLR ligands.

HEK001 cells were pretreated with Stattic or vehicle, then challenged with bacterial lipopolysaccharide (LPS), peptidoglycan (PGN) or S. typhimurium flagellin (FLA). TNF-α (A) and IL-β (B) in supernatants was assessed by ELISA at 24 and 48 hours. C) HEK001 cells treated with vehicle or Stattic were infected with ACAM-2000 at MOI  = 20. Secreted TNF-α, IL-1β, and IL-6 were assessed at 12 hours (n = 2 wells per condition). Representative of triplicate experiments is shown. Asterisk, p≤0.05.