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. 2014 Nov 24;9(11):e113690. doi: 10.1371/journal.pone.0113690

Figure 8. Stattic reduces STAT3 and TAK1 detection in cytosol of infected keratinocytes.

Figure 8

Left: HEK001 keratinocytes were uninfected, or infected for 3 hours with ACAM-2000 at 20 MOI, in the presence of DMSO, Stattic, or Nec-1. At 3 hours cells were collected in cold hypotonic buffer. Left: Cytoplasmic fractions were evaluated for STAT3, TAK1, RIP1K, and housekeeping protein β-actin by immunoblot. Right: Relative intensity of STAT3 and TAK1 protein bands in infected, Stattic-treated wells, compared with infected, DMSO treated wells (n = 2 experiments). Asterisk, p≤0.05.