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. 2014 Nov 24;9(11):e113650. doi: 10.1371/journal.pone.0113650

Figure 4. Polymerization of full-length OPN and the N-terminal part of OPN.

Figure 4

The N-terminal part of recombinant OPN (A), full-length recombinant OPN (B–C) and human milk OPN (D) were incubated with TG2 in a 10∶1 ratio (w/w). In A and B, proteins were separated by 18% SDS-PAGE and stained with Coomassie Brilliant blue. In C and D, proteins were separated by 10% SDS-PAGE and detected by a polyclonal OPN antibody. A, B and D; OPN without TG2 (lane 1), or TG2-treated for 1 h (lane 2), 3 h (lane 3) and 16 h (lane 4). C, OPN without TG2 (lane 1), or TG2-treated for 15 min (lane 2), 30 min (lane 3), 1 h (lane 4), 3 h (lane 5) and 16 h (lane 6).