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. 2014 Nov 13;20:2269–2274. doi: 10.12659/MSM.892413

Table 1.

Primer sequences and reaction conditions for MMP polymorphism detection.

Gene polymorphim size (bp) Primer sequence Annealing temperature Restriction enzyme Product
MMP1 −1607 1G/2G F: TGACTTTTAAAACATAGTCTATGTTCA 51°C AluI 2G: 269
R: TCTTGGATTGATTTGAGATAAGTCATAGC 1G: 241 +28
MMP3 −1612 5A/6A F: GGTTCTCCATTCCTTTGATGGGGGGAAAGA 65°C TthIII 6A: 130
R: CTTCCTGGAATTCACATCACTGCCACCACT 5A: 96 +34
MMP9 −1562C/T F: ATGGCTCATGCCCGTAATC 62°C SphI C: 352
R: TCACCTTCTTCAAAGCCCTATT T: 208 +144