Table 1.
Gene polymorphim size (bp) | Primer sequence | Annealing temperature | Restriction enzyme | Product |
---|---|---|---|---|
MMP1 −1607 1G/2G | F: TGACTTTTAAAACATAGTCTATGTTCA | 51°C | AluI | 2G: 269 |
R: TCTTGGATTGATTTGAGATAAGTCATAGC | 1G: 241 +28 | |||
MMP3 −1612 5A/6A | F: GGTTCTCCATTCCTTTGATGGGGGGAAAGA | 65°C | TthIII | 6A: 130 |
R: CTTCCTGGAATTCACATCACTGCCACCACT | 5A: 96 +34 | |||
MMP9 −1562C/T | F: ATGGCTCATGCCCGTAATC | 62°C | SphI | C: 352 |
R: TCACCTTCTTCAAAGCCCTATT | T: 208 +144 |