Fig. 1.

a Effect of AZ64 on Trk phosphorylation induced by ligands. SY5Y cells were transfected with either TrkA (SY5Y-TrkA) or TrkB (SY5Y-TrkB), and cells were exposed to ligands (NGF or BDNF) in the absence and presence of increasing concentrations of AZ64. Maximal inhibition of ligand-induced autophosphorylation was seen by 30–100 nM. 1A. b Effect of AZ64 on growth of SY5Y cells (Trk-null) in medium with 2 % serum. Cells were cultured in RT-CES growth plates in the presence of BDNF. AZ64 from 3 to 300 nM had no effect on the growth of SY5Y cells in presence of BDNF, as assessed by RT-CES analysis. c Effect of AZ64 on growth of SY5Y-TrkB cells in medium with 2 % serum. Cells were cultured in RT-CES growth plates in the presence of BDNF and increasing concentrations of AZ64. There was a dose-dependent inhibition of growth, with an IC₅₀ between 30 and 100 nM