Table 2.
Microarray profile of expression of TLR pathway associated gene mRNAs in BMØ from Wt, MyD88-KO and TrifLps2 mice of 2-months of age in response to P. gingivalis.
| Gene symbol | Gene | Fold change (Mean) | ||
|---|---|---|---|---|
| Wt | MyD88-KO | TrifLps2 | ||
| Il6 | Interleukin 6 | 1298.64 | 3.26 | 836.68 |
| Csf3 | Colony stimulating factor 3 (granulocyte) | 836.98 | 1.08 | 724.50 |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | 812.55 | 9.67 | 381.26 |
| Il1a | Interleukin 1 alpha | 746.41 | 6.25 | 687.91 |
| Il1b | Interleukin 1 beta | 736.05 | 0.69 | 654.97 |
| Clec4e | C-type lectin domain family 4, member e | 72.48 | 14.16 | 49.64 |
| Il12a | Interleukin 12A | 61.84 | 0.69 | 53.47 |
| Ccl2 | Chemokine (C-C motif) ligand 2 | 48.50 | 6.31 | 45.73 |
| Il10 | Interleukin 10 | 33.68 | 0.47 | 6.91 |
| Il1r1 | Interleukin 1 receptor, type I | 21.99 | 0.96 | 12.24 |
| Tnf | Tumor necrosis factor | 16.69 | 4.58 | 10.58 |
| Lta | Lymphotoxin A | 14.82 | 1.48 | 8.14 |
| Csf2 | Colony stimulating factor 2 (granulocyte-macrophage) | 10.46 | 0.69 | 3.43 |
| Ifnb1 | Interferon beta 1, fibroblast | 8.98 | 17.07 | 4.26 |
| Ifng | Interferon gamma | 8.45 | 0.69 | 0.68 |
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | 8.04 | 19.41 | 2.45 |
| Nfkbia | Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha | 6.38 | 1.91 | 3.98 |
| Tnfaip3 | Tumor necrosis factor, alpha-induced protein 3 | 6.33 | 3.60 | 4.90 |
| Cd14 | CD14 antigen | 5.25 | 1.21 | 4.98 |
| Cebpb | CCAAT/enhancer binding protein (C/EBP), beta | 4.43 | 1.20 | 2.63 |
| Ube2v1 | Ubiquitin-conjugating enzyme E2 variant 1 | 3.92 | 0.90 | 0.88 |
| Tlr2 | Toll-like receptor 2 | 3.04 | 2.04 | 3.17 |
| Tlr1 | Toll-like receptor 1 | 2.84 | 0.97 | 2.33 |
| Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | 2.77 | 7.12 | 2.38 |
| Hspa1a | Heat shock protein 1A | 2.44 | 2.48 | 0.98 |
| Peli1 | Pellino 1 | 2.14 | 1.30 | 0.68 |
| Il6ra | Interleukin 6 receptor, alpha | 0.45 | 0.41 | 0.27 |
| Pglyrp1 | Peptidoglycan recognition protein 1 | 0.38 | 0.83 | 0.46 |
| Tlr9 | Toll-like receptor 9 | 0.36 | 0.48 | 0.17 |
| Tlr8 | Toll-like receptor 8 | 0.34 | 0.50 | 0.29 |
| Ly86 | Lymphocyte antigen 86 | 0.22 | 0.38 | 0.08 |
| Tlr5 | Toll-like receptor 5 | 0.07 | 0.28 | 0.12 |
BMØ from Wt, MyD88-KO, and TrifLps2 mice of 2-months of age were stimulated with P. gingivalis (MOI 100) for 24h. mRNA expression was normalized to Actb mRNA expression levels. Mean fold change in the mRNA expression in response to P. gingivalis stimulation in comparison with un-stimulated control was determined by ΔΔCt method. Data presented as mean value of 3 independent experiments using individual mice.