Table 3.

Expression of TLR pathway associated gene mRNAs in BMØ from Wt and MyD88-KO mice with 2-months of age to P. gingivalis.

Gene symbol	Gene	Fold change (Mean)		P value#
		Wt	MyD88-KO
Csf3	Colony stimulating factor 3 (granulocyte)	837	1.08	0.01
Il1r1	Interleukin 1 receptor, type I	21.99	0.96	0.002
Csf2	Colony stimulating factor 2 (granulocyte-macrophage)	10.45	0.69	0.04
Cxcl10	Chemokine (C-X-C motif) ligand 10	8.03	19.41	0.03
Cebpb	CCAAT/enhancer binding protein (C/EBP), beta	4.42	1.19	0.03
Tlr1	Toll-like receptor 1	2.83	0.96	0.0001
Tlr6	Toll-like receptor 6	1.95*	0.93	0.01
Hrb	HIV-1 Rev binding protein	1.93*	1	0.02
Hras1	Harvey rat sarcoma virus oncogene 1	1.9*	1.19	0.02
Tnfrsf1a	Tumor necrosis factor receptor superfamily, member 1a	1.49*	0.93	0.02
Hsp90ab1	Heat shock protein 90 alpha (cytosolic), class B member 1	1.46*	1.03	0.04
Ly96	Lymphocyte antigen 96	1.41*	0.72	0.008
Nfkb1	Nuclear factor of kappa light chain gene enhancer in B-cells	1.38*	0.77	0.01
Map2k4	Mitogen activated protein kinase kinase 4	1.32*	0.9	0.01
Myd88	Myeloid differentiation primary response gene 88	1.31*	0.7	0.01
Chuk	Conserved helix-loop-helix ubiquitous kinase	1.26*	0.97	0.02
Ticam2	Toll-like receptor adaptor molecule 2	1.18*	0.76	0.01
Irf1	Interferon regulatory factor 1	0.9*	1.66	0.04
Casp8	Caspase 8	0.7*	0.78	0.008
Fos	FBJ osteosarcoma oncogene	0.57*	1.12	0.002
Tlr5	Toll-like receptor 5	0.06*	0.27	0.004

BMØ from Wt and MyD88-KO mice of 2-months of age were stimulated with P. gingivalis (MOI 100) for 24h. Expression was normalized to Actb mRNA expression levels. Mean fold change in the mRNA expression to P. gingivalis stimulation in comparison with un-stimulated control was determined by ΔΔCt method. Comparisons between groups were performed using Student T-test. Data presented as mean value of 3 independent experiments using individual mice.

^*= mRNA expression did not meet the 2-fold criteria.

^#= P<0.05; fold change in the mRNA expression compared between Wt and MyD88-KO groups.