Table 4.
Microarray profiling of expression of TLR pathway associated gene mRNAs in BMØ from Wt, MyD88-O and TrifLps2 mice of 12- months of age in response to P. gingivalis.
| Gene symbol | Gene | Fold change (Mean) | ||
|---|---|---|---|---|
| Wt | MyD88-KO | TrifLPS2 | ||
| Il1b | Interleukin 1 beta | 4344.65 | 1.01 | 3299.31 |
| Csf3 | Colony stimulating factor 3 (granulocyte) | 2713.51 | 0.47 | 2951.98 |
| Il1a | Interleukin 1 alpha | 2525.22 | 8.57 | 4134.11 |
| Il6 | Interleukin 6 | 2231.90 | 5.45 | 2888.33 |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | 244.92 | 1.63 | 128.69 |
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | 194.58 | 48.95 | 1.64 |
| Il1r1 | Interleukin 1 receptor, type I | 65.04 | 0.55 | 5.48 |
| Il6ra | Interleukin 6 receptor, alpha | 57.23 | 0.49 | 0.27 |
| Clec4e | C-type lectin domain family 4, member e | 53.09 | 23.63 | 113.89 |
| Il12a | Interleukin 12A | 42.55 | 0.85 | 79.49 |
| Rel | Reticuloendotheliosis oncogene | 41.56 | 1.12 | 0.88 |
| Ccl2 | Chemokine (C-C motif) ligand 2 | 40.22 | 5.27 | 58.74 |
| Cd14 | CD14 antigen | 19.73 | 0.79 | 4.41 |
| Csf2 | Colony stimulating factor 2 (granulocyte-macrophage) | 18.90 | 1.18 | 36.77 |
| Il10 | Interleukin 10 | 10.49 | 0.81 | 17.24 |
| Tnf | Tumor necrosis factor | 8.63 | 3.77 | 26.04 |
| Tnfaip3 | Tumor necrosis factor, alpha-induced protein 3 | 5.94 | 3.68 | 8.41 |
| Lta | Lymphotoxin A | 5.84 | 1.67 | 11.00 |
| Ticam1 | Toll-like receptor adaptor molecule 1 | 5.19 | 1.03 | 1.15 |
| Nfkbia | Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha | 3.30 | 2.03 | 6.31 |
| Il2 | Interleukin 2 | 2.80 | 0.85 | 0.89 |
| Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | 2.09 | 1.48 | 1.93 |
| Tlr2 | Toll-like receptor 2 | 2.07 | 1.92 | 2.58 |
| Irf1 | Interferon regulatory factor 1 | 0.50 | 1.72 | 0.80 |
| Map2k4 | Mitogen activated protein kinase kinase 4 | 0.50 | 0.94 | 1.05 |
| Gusb | Glucuronidase, beta | 0.48 | 1.18 | 0.90 |
| Tlr3 | Toll-like receptor 3 | 0.46 | 1.92 | 0.38 |
| Casp8 | Caspase 8 | 0.45 | 0.95 | 0.82 |
| Ppara | Peroxisome proliferator activated receptor alpha | 0.45 | 0.85 | 0.89 |
| Map3k7 | Mitogen activated protein kinase kinase kinase 7 | 0.44 | 0.93 | 1.00 |
| Btk | Bruton agammaglobulinemia tyrosine kinase | 0.43 | 0.84 | 0.71 |
| Tlr7 | Toll-like receptor 7 | 0.42 | 0.70 | 0.60 |
| Ube2n | Ubiquitin-conjugating enzyme E2N | 0.42 | 0.66 | 0.73 |
| Nr2c2 | Nuclear receptor subfamily 2, group C, member 2 | 0.41 | 0.45 | 0.51 |
| Pglyrp1 | Peptidoglycan recognition protein 1 | 0.40 | 0.76 | 0.73 |
| Hmgb1 | High mobility group box 1 | 0.40 | 0.68 | 0.77 |
| Eif2ak2 | Eukaryotic translation initiation factor 2-alpha kinase 2 | 0.39 | 1.63 | 0.60 |
| Tlr4 | Toll-like receptor 4 | 0.38 | 1.24 | 0.76 |
| Irak1 | Interleukin-1 receptor-associated kinase 1 | 0.37 | 1.01 | 0.79 |
| Map2k3 | Mitogen activated protein kinase kinase 3 | 0.37 | 0.73 | 0.49 |
| Hprt1 | Hypoxanthine guanine phosphoribosyl transferase 1 | 0.36 | 0.66 | 0.70 |
| Fadd | Fas (TNFRSF6)-associated via death domain | 0.31 | 1.16 | 0.75 |
| Map3k1 | Mitogen activated protein kinase kinase kinase 1 | 0.28 | 0.73 | 0.40 |
| Fos | FBJ osteosarcoma oncogene | 0.26 | 0.81 | 0.24 |
| Tlr8 | Toll-like receptor 8 | 0.23 | 0.50 | 0.20 |
| Ly86 | Lymphocyte antigen 86 | 0.15 | 0.35 | 0.05 |
| Tlr9 | Toll-like receptor 9 | 0.13 | 0.51 | 0.10 |
| Tlr5 | Toll-like receptor 5 | 0.10 | 0.11 | 0.02 |
BMØ from Wt, MyD88-KO and TrifLps2 mice of 12-months of age were stimulated with P. gingivalis (MOI 100) for 24h. Expression was normalized to Actb mRNA expression levels. Mean fold change in the mRNA expression in response to P. gingivalis stimulation in comparison with un-stimulated control was determined by ΔΔCt method. Data presented as mean value of 3 independent experiments using individual mice.