Table 5.

Expression of TLR pathway associated gene mRNAs in BMØ from Wt and Trif^Lps2 mice of 2-months of age to P. gingivalis

Gene symbol	Gene	Fold change (Mean)		P value#
		Wt	TrifLps2
Il1r1	Interleukin 1 receptor, type I	21.99	12.23	0.049
Tlr1	Toll-like receptor 1	2.84	2.33	0.014
Tlr6	Toll-like receptor 6	1.96*	1.27	0.044
Hras1	Harvey rat sarcoma virus oncogene 1	1.9*	1.34	0.033
Tnfrsf1a	Tumor necrosis factor receptor superfamily, member 1a	1.49*	0.9	0.029
Ly96	Lymphocyte antigen 96	1.41*	0.86	0.03
Nfkb1	Nuclear factor of kappa light chain gene enhancer in B-cells 1, p105	1.39*	0.88	0.035
Map2k4	Mitogen activated protein kinase kinase 4	1.32*	0.99	0.033
Chuk	Conserved helix-loop-helix ubiquitous kinase	1.27*	0.91	0.034
Rela	V-rel reticuloendotheliosis viral oncogene homolog A (avian)	0.98*	0.69	0.007
Tirap	Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein	0.98*	0.55	0.034
Hmgb1	High mobility group box 1	0.89*	0.64	0.042

BMØ from Wt and Trif^Lps2 mice of 2-months of age were stimulated with P. gingivalis (MOI 100) for 24h. Expression of genes was normalized using Actb. Mean fold change in the mRNA expression to P. gingivalis stimulation in comparison with un-stimulated control was determined by ΔΔCt method. Comparisons between groups were performed using Student t-test. Data presented as mean value of 3 independent experiments using individual mice.

^*= mRNA expression did not meet the 2-fold criteria.

^#= P<0.05; fold change in the mRNA expression compared between Wt and Trif^Lps2 groups.