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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Transl Res. 2014 May 24;164(5):359–365. doi: 10.1016/j.trsl.2014.05.011

Figure 3. . In vitro demonstration of intercellular tumor-tumor and tumor-stromal interaction via TnTs in ovarian cancer.

Figure 3

Ovarian cancer SKOV3 cells also formed TnTs to each other and to IOSE cells in co-culture. TnTs connecting DiO-labeled SKOV3 human ovarian cancer cells (green) with DiI-labeled IOSE human ovarian epithelial cells (red) are demonstrated in the following panels. (A) Brightfield image; and (B) same frame imaged using TRITC filter. Cells were labeled using the lipophilic dyes DiI (red) and DiO (green) per the manufacturer’s protocol and per our prior demonstration using these dyes to visualize TnTs with fluorescence microscopy[4]. In cases of co-culture, cells were cultured in a 1:1 ratio. These in vitro data confirmed that TnTs form tumor-tumor and tumor-stromal cell connections in both osteosarcoma and ovarian cancer.