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SKOV3 cells transfected with Alexa-Fluor-88-tagged miR-199a were cultured in a low-serum, hyperglycemic culture medium.
A) Multiple TnTs connecting SKOV3 cells expressing fluorescing miR-199a.
B) Demonstration of a long TnT connecting a miR-199a-transfected SKOV3 cell (green) to a DiI-marked IOSE (red) ovarian epithelial cell, with intercellular transport of miR-199a in progress.
