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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Transl Res. 2014 May 24;164(5):359–365. doi: 10.1016/j.trsl.2014.05.011

Figure 7. FACS analysis of cells grown in open culture vs. separated by membrane filter in modified invasion chambers.

Figure 7

Adapting our model of in vitro cell culture of osteosarcoma cells and osteoblast cells, we used modified Boyden chambers including membrane filters with the smallest commercially available pore-size (0.4 μm, or 400 nm). At 48 hours, cells in the bottom chambers were collected and sorted using fluorescence-activated cell sorting (FACS); this step separated out single-positive non-recipient cells that were excluded from further analysis. (A) FACS analysis of double-positive MC3T3 cells following transwell assay for 48 hours, versus (B) FACS analysis of double-positive MC3T3 cells in open co-culture with K7M2 for 48 hours (without membrane barrier separation). The difference in these results was statistically significant with a p-value of 0.0002; the standard deviation was 2.15 for the transwell plate and 3.50 for the open culture experiments.