Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 24;207(4):499–516. doi: 10.1083/jcb.201408025

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Jones et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Loss of LICs does not affect dynein complex stability. (A) LIC KnD in HeLaM cells was assessed by immunoblotting with isoform-specific LIC antibodies (asterisk, a cross-reacting band; arrow, LIC2) with lamin A/C depletion (LA) as a control. Black line indicates that intervening lanes have been spliced out. Tub, tubulin. (B) Extracts made from untreated (wild type [WT]) or HeLaM cells depleted of lamin A/C, both LICs (LIC), or DHC (HC), were immunoblotted for IC and DHC, with tubulin as a loading control. (C) KI-treated extracts from control (cont.; top) or LIC1- and 2-depleted (bottom) HeLaM cells were run on sucrose gradients containing 0, 100, or 150 mM KI and immunoblotted for IC. (D) Silver-stained SDS-PAGE of dynein isolated from control and LIC1- and 2-depleted cells. Molecular mass markers (in kilodaltons) are shown.