Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 24;207(4):499–516. doi: 10.1083/jcb.201408025

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Jones et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — LICs 1 and 2 act redundantly in bipolar spindle assembly in HeLaM cells. (A) HeLaM cells depleted of both LICs (LIC1&2) or DHC were labeled with anti–α-tubulin and DAPI. Maximum projections of deconvolved z series are shown. Cyan arrows show major poles; yellow arrowheads show minor poles. Bar, 5 µm. (B–D) Quantitation of HeLaM spindle morphology (see Results for details). (B) Both LICs, DHC, or Lamin A/C (LA) were depleted. (C) KnDs with scrambled (SC), LIC1, LIC2, or both LIC1 and 2 siRNAs. For B and C, ≥100 cells were scored per condition; the mean of three independent experiments ± SEM is shown. (D) HeLaM cells were depleted of both LICs for 48 h and then transfected RNAi-resistant LIC1-mKate or LIC2-mKate, with mKate as a control (115, 112, and 235 cells were scored, respectively, from three independent experiments; means ± SEM). Two-way ANOVA results versus controls: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.