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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Cell. 2014 Oct 30;159(4):884–895. doi: 10.1016/j.cell.2014.09.055

Figure 1. Abnormal gene expression of SCNT embryos at the 1- and 2-cell stage.

Figure 1

(A) Schematic illustration of the experimental approach. Samples used for RNA-seq are marked by dashed rectangles.

(B, C) Scatter plots comparing gene expression levels between IVF and SCNT embryos at the 1-cell stage (B) and the 2-cell stage (C). Genes expressed higher in IVF embryos (FC > 3.0, IVF-high) and higher in SCNT embryos (FC > 3.0, SCNT-high) are colored with red and blue, respectively.

(D) Heatmap illustration showing differentially expressed genes (DEGs) (FC > 5.0, FPKM > 5 in each replicates) obtained by a pairwise comparison between donor cumulus cells, IVF 2-cell and SCNT 2-cell embryos. A total of 3775 DEGs are classified into 5 groups by unsupervised hierarchical clustering.

(E) Gene ontology analysis of the 5 groups classified in (D).

See also Figure S1.