Figure 7. A model illustrating how the H3K9me3 reprogramming barrier can be overcome.
Suv39h-deposited H3K9me3 in somatic cells serves as a transcriptional barrier for SCNT-mediated reprogramming which affects normal embryonic development (Left). Removal of this barrier either by the expression of exogenous Kdm4d (Middle) or by prevention of H3K9me3 establishment by Suv39h knockdown (Right) can lead to activation of developmental regulators in SCNT embryos, resulting in successful embryonic development.