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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Cell. 2014 Nov 6;159(5):1070–1085. doi: 10.1016/j.cell.2014.10.031

Figure 4. Tnfr2 induces jagged1a in endothelial cells, encouraging HSC specification.

Figure 4

(A) kdrl:mcherry+ cells from dissected trunks of Std or Tnfr2 morphants were purified by FACS at 28hpf for qPCR. Levels of indicated transcripts along x-axis are shown relative to the housekeeping gene ef1a. Bars represent means ± S.E.M. of duplicate samples. (B) AGM regions from fli1a:Gal4; UAS:Tnfr2 embryos were dissected and subjected to qPCR for transcripts shown along x-axis. Bars represent means ± S.E.M. of expression relative to the housekeeping gene rps11. (C) Std (top panels) or Jag1a (bottom panels) morphants were interrogated for runx1 expression at 26hpf and efnb2a and dlc at 28hpf by WISH. Numbers represent larvae with indicated phenotype. (D) Enumeration of runx1+ cells in Tnfr2 and/or Jag1a morphants at 28hpf. (E) Enumeration of runx1+ cells in Tnfr2 and/or Notch1a and/or Notch1b morphants at 28hpf. Each dot is the number of HSCs per embryo, and red lines indicate means ± S.E.M. (D–E) ***p<0.001; ns, not significant. See also Supplementary Figure 3.