Figure 3. SYMRK mediates spontaneous AM-related signal transduction.
Hairy roots of L. japonicus Gifu wild-type (A) or a stable transgenic L. japonicus MG20 reporter line carrying a SbtM1 promoter:GUS fusion (pSbtM1:GUS) (B) transformed with the empty vector (EV), pUB:NFR1-mOrange (NFR1), pUB:NFR5-mOrange (NFR5), or pUB:SYMRK-mOrange (SYMRK) were generated. (A) Relative expression of SbtM1 or Bcp1 at 40 dpt was determined in three biological replicates for each treatment via qRT-PCR. Transcript levels in each replicate were determined through technical duplicates. Expression was normalized with the house keeping genes EF1alpha and Ubiquitin. Circles indicate expression relative to the EF1alpha gene. Dashed circles indicate that no transcripts could be detected for this sample. Samples in which the indicated transcript could not be detected were floored to 1. A Dunnett's test was performed comparing the transcript levels of Bcp1 detected for each treatment with those detected in the empty vector samples. Stars indicate significant differences. **, p < 0.01. (B) GUS activity was analysed by histochemical staining with X-Gluc 40 and 60 dpt. Representative root sections are shown. Number of plants with detectable GUS activity per total plants is indicated. Bars, 500 μm.