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. 2014 Nov 18;5:5399. doi: 10.1038/ncomms6399

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(a,b) Pre-steady-state kinetics of ubiquitin dissociation (a) and association (b) for USP4 CD (upper panel) and FL (lower panel) measured by fluorescence polarization in a stopped-flow device. (c) Equilibrium binding of ubiquitin to USP4 CD (upper panel) and FL (lower panel) measured by fluorescence polarization (error bars represent s.e.m. for at least three independent replicates).