Table 2. Enzymatic and kinetic parameters of USP4 and USP15 constructs.
| kcat (s−1) | KM (μM) | kslow (s−1) | kfast (s−1) | kon (μM−1 s−1) | KD (μM) | |
|---|---|---|---|---|---|---|
| USP4 FL isof. 1 | 0.30±0.07 | 0.15±0.05 | 0.053±0.003 (36%) | 0.55±0.016 (64%) | 5.1±0.23 | 0.092±0.021 |
| USP4 CD | 0.0013±0.00001 (90%) | 0.011±0.0008 (10%) | 0.074±0.017 | 0.044±0.007 | ||
| USP4 FL isof. 2 | 0.29±0.06 | 0.20±0.05 | ||||
| USP4 FL Δinsert | 0.042±0.002 | 0.16±0.09 | ||||
| USP4 FL F386G | 0.13±0.02 | 0.25±0.03 | 0.024±0.002 (30%) | 0.26±0.010 (70%) | ||
| USP4 CD F386G | 0.027±0.002 | 0.16±0.04 | 0.015±0.0006 (79%) | 0.12±0.016 (21%) | 0.035±0.007 | ~1 |
| USP4 FL PQ385AV | 0.092±0.007 | 0.14±0.01 | ||||
| USP4 D1D2 | 0.030±0.004 | 0.086±0.030 | ||||
| USP15 FL | 0.15±0.004 | 0.20±0.03 | ||||
| USP15 CD | 0.05±0.004 | 0.10±0.02 |
USP, ubiquitin-specific protease.