Figure 2. Autonomous oxidation of Ero1α by oxygen and H₂O₂.

Reduced Ero1α (2 μM) was incubated either under anaerobic conditions or with increasing concentrations of oxygenated buffer (A) or H₂O₂ (C). The redox state of Ero1α was frozen by alkylating with NEM at the given time points (annotated in seconds), before analysing the samples by non-reducing SDS/PAGE and silver staining. The positions of the fully reduced (red) and oxidized (ox) forms are as indicated. (B) The fraction of Ero1α re-oxidized in the presence of oxygen for three independent experiments was quantified and the mean value calculated and plotted against the time of incubation (error bars represent S.D.).