Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) KEA3.2 has an amino-terminal chloroplast-targeting peptide, a cation/proton exchanger (CPA2) domain and a putative KTN domain. (b) Differential interference contrast, GFP and chlorophyll fluorescence were imaged in leaf guard cells from Arabidopsis WT plants and kea3-1 plants transformed with KEA3.2-GFP. Scale bar, 10 μm. (c–e) KEA3 abundance was measured by western blot in: (c) Total protein (TP) and chloroplast (Ch) protein extracts; (d) Ch subfractionated into envelope (En), stroma (St) and thylakoids (Th); (e) Th separated into the fraction pelleted by 1,000g (1 K), grana (Gr), intermediate membranes (IM) and stromal lamellae (SL). (c–e) Ponceau red (P.R.) stains of membranes after protein transfer before immunodetection are shown as loading controls.