Table 2. Catalytic parameters.
Vmax, maximum catalytic velocity; Km, Michaelis-Menten constant; specific activity (SA), amount of active enzyme from the total protein quantity; kcat, number of substrate molecules that are transformed per active site and per time unit. kcat/Km defines the catalytic efficiency.
| Enzyme | Vmax (nmol·min−1) | Km (mM) | kcat (s−1) | kcat/Km (M−1·s−1) × 104 | SA (μM·min−1·mg−1) |
|---|---|---|---|---|---|
| BaKynB | 65.41±2.64 | 0.40±0.05 | 50.56 | 12.64 | 130.82 |
| BcKynB | 58.15±0.98 | 0.57±0.02 | 43.94 | 7.71 | 116.30 |
| PaKynB-His | 147.99±8.42 | 0.98±0.13 | 114.21 | 11.65 | 295.98 |