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. 2014 Nov 18;14:294. doi: 10.1186/s12870-014-0294-3

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© van Wyk et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression changes of cystatins, cysteine proteases and vacuolar processing enzymes. (A) Expression of cystatins (CYS) (B) cysteine proteases (CYP) and (C) vacuolar processing enzymes (VPE) in 4, 8 and 14 week old nodules expressed as FPKM (transcript abundances in fragments per kilobase of exon per million fragments mapped). Colour scale represents transcription for each time point normalized by subtracting the mean/median of three values from each individual value for each gene reduced by SD/RMS. * indicates significant change (p < 0.05) in transcription between individual time points. Multi-experiment viewer (MeV v4.8.1) software package was applied to graphically represent data [52].