Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Jun 20;7(3):293–306. doi: 10.1007/s12195-014-0342-y

Figure 4. Experimental approach to quantify the migration transit times of the nucleus through the narrow constrictions.

Figure 4

Time-lapse image sequences were used to measure the amount of time between when the cell nucleus enters and exits the constriction. In this example, a Lmna+/+ mouse embryonic fibroblast is shown. Entry into the constriction is defined as the time point at which the cell nucleus crosses a threshold (left yellow dotted line) located 5 μm in front of the constriction center; the nucleus is defined as having exited the constriction when the back of the nucleus crosses a threshold 5 μm past the center of the constriction (right yellow dotted line). The white dashed line indicates the outline of the nucleus for demonstration purposes. The center of the constriction is marked with a red dashed line. See Supplemental Data for representative time-lapse microscopy sequences of Lmna+/+, Lmna+/− and Lmna−/− cells. Scale bar: 20 μm.