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. 2014 Nov 18;21(1):99. doi: 10.1186/s12929-014-0099-6

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© Lin et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Balance of CCR2+ inflammatory monocytes between the BM and lung. (A-F) PBMC and BM cells were harvested from infected mice at day 7 post-infection. (A-B) The dot plots and percentage of Gr1 + CD11b + cells in PBMC are shown. This data are a composite of three independent experiments (n = 9 mice per group, mean ± SEM, *P < 0.05; ** P < 0.01). (C-D) The dot plots and percentage of Ly6C^highCCR2+ monocytes in the Gr1 + CD11b + gated cells in PBMC are shown. This data are a composite of two independent experiments (n = 3 mice per group, mean ± SEM, ***P < 0.001). (E-F) The dot plots and numbers of CCR2+ monocytes were counted from indicated virus infections at day 7 post-infection. These data are a composite of four independent experiments (mean ± SEM; *P < 0.05; ***P < 0.001). (G-H) CFSE-labeled monocytes of BM were transferred into naïve or day 4 post-infected mice. After 2 days, infiltrating leukocytes were harvested from the lungs and stained with a specific Ab against CCR2. (G) The percentage of CCR2 + CFSE + transferred cells is shown for the groups indicated. (H) Absolute numbers of transferred CCR2 + CFSE + monocytes in the lungs were counted in each group. These data are a composite of three independent experiments (n = 7 to 8 mice per group, means ± SEM; ns: no significant difference; ***P < 0.001). (I) Percentage of Gr1 + CD11b + cells in PBMC or lung is shown. (J) Ly6C^highCCR2+ inflammatory monocytes in Gr1 + CD11b + gated population in PBMC or lung are shown. This is a representative result from three repeated experiments.